■ 基本信息
啟動子: | CMV |
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質粒分類: | 病毒系列，慢病毒克隆載體 |
原核抗性: | Amp |
克隆菌株: | Stbl3 |
培養條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導方式: | 無須誘導 |
5'測序引物: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
3'測序引物: | 根據序列設計引物 |
■ 質粒屬性
質粒宿主: | 哺乳細胞,慢病毒 |
質粒用途: | 蛋白表達 |
片段類型: | ORF |
片段物種: |
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原核抗性: | Amp |
真核抗性: |
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熒光標記: | 綠色
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■ 質粒簡介
pCDH-CMV-MCS-EF1-copGFP是一個慢病毒表達質粒。The puromycin-resistance gene (puro) was cloned into pCDH-EF1-MCS-T2A-copGFP (A) and pCDH-MCS-T2A-copGFP-MSCV (B); and the copGFP gene (cG) was cloned into pCDH-EF1-MCS-T2A-Puro (C) and pCDH-MCS-T2A-Puro-MSCV (D). The resulting dual reporter constructs were packaged into pseudoviral particles followed by transduction into HT1080 cells. All constructs were also puromycin resistant (data not shown)。
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK780pCDH-CMV-MCS-EF1-copGFP慢病毒表達質粒.txt
